Fig 1: MPs effect on THP1 cell line. (A) Quantification of MP uptake by THP-1 cells using confocal microscopy after 3 hours of exposure. Scale bar: 15 µm. (B) Real-time qPCR of the relative quantification of THP1 genes under control of G-CSF, GM-CSF, IL-16, IL-2 and IL-1RA pathways following 48 h of MP treatment from healthy controls (green), diabetics with neuropathy (orange) or CF (purple) patients. Relative transcript levels are represented as the ratios between the 2 subpopulations of their 2−ΔΔCp real-time PCR values. (C) THP1 differentiation into osteoclasts with M-CSF and sRANKL in the presence (bottom picture) or absence (top picture) of CF-derived MPs. Scale bar: 250 µm. the plot represents the quantitative comparison between the percentage of TRAcP-positive cells (multinucleated osteoclast-like cells) formed in cultures with CF-derived MPs alone (grey bar) or with M-CSF and sRANKL (black bar) or with with M-CSF, sRANKL and CF-derived MPs (hatched bar). The error bar represents the difference between three independent experiments. (D). Monocytes differentiated into osteoclast in presence of MPs. Monocytes were sorted from two healthy patients. Osteoclast differentiation was done with M-CSF (25 ng/ml) and sRANKL (100 ng/ml) replaced every other day in presence of MPs from healthy patients (top picture), DN patient (Middle picture) or CF patient (bottom picture). Scale bar: 150 µm. The number of osteoclast was counted on 40 fields for each conditions and MPs from three patients for each group had been used. The plot represents the quantitative comparison between the percentage of TRAcP-positive cells (multinucleated osteoclast-like cells) formed in cultures with healthy- (green), DN- (orange) or CF-derived (purple) MPs compare d to the control (Ø MPs). *p < 0.05, **p < 0.01 or ***p < 0.001.